Development and Characterization of Osimertinib Loaded Ethylcellulose Based Sustained Release Nanosponges: Cytotoxicity and Biocompatibility Studies

Abstract

Osimeritininb loaded nanosponges were prepared by ultrasonication technique using ethylcellulose and polyvinyl alcohol. Nanosponges (OS1-OS6) exhibited particle size, PDI, and zeta potential, within
the range of (309.4±1.23 to 535.1±2.15 nm), (0.215±0.057 to 0.616±0.081) and (-3.11±0.036 to -4.44±0.057
mV). In contrast, entrapment efficiency and drug loading were found in the range of (51.45±2.12% to
83.36±2.21%) and (6.14±1.43%-16.17±2.12%), respectively. FTIR, DSC, XRD, and SEM studies of optimized OS4 showed that osimertinib was physically entangled in an amorphous state within the porous state
of the nanosponge. OS4 demonstrated 92.14 ±1.01% drug release in a sustained manner for 48 h. The results
of cytotoxicity and haemolytic studies revealed OS4 as a potential anticancer and biocompatible nanocarrier.
Moreover, the stability study indicated OS4 has relatively similar drug release at different accelerated temperature conditions. In conclusion, osimertinib loaded nanosponges (OS4) could be efficient for the effective
treatment of non-small cell lung cancer.