Exploring Anti-Oxidant, Anti-Inflammatory Activity And Toxicological Evaluation Of Curcumin Pyrazole Analogue

Authors

  • Manivel Kannan, Ramasamy Thilagavathi*

Keywords:

Antioxidant, Curcumin pyrazole analogue, DPPH assay, Anti-inflammatory activity, Acute toxicity.

Abstract

Curcumin is a traditional herb in India, even though its uses in the traditional medicine for antioxidant, anti-inflammation, antitumor, antiviral and Alzheimer’s disease, it will not be used full-fledged clinically due to the inefficient bioavailability profile in the physiological system. It stimulates many scientists to develop a semi synthetic analogue from herbal sources that was opt to the pathological target. The aim of the present study was to evaluate the anti-oxidant ,anti-inflammatory activity and acute toxicity  of  the  curcumin pyrazole analogue .The anti-oxidant activity of the curcumin pyrazole analogue was found  by DPPH anti-oxidant assay,  anti-inflammatory activity by carrageenan-induced paw edema model and acute oral toxicity by following the OECD 423 guidelines. The dose of the curcumin pyrazole analogue for anti-inflammatory activity was selected based on the results of acute toxicity and there was a dose dependent inhibition of paw oedema was seen in the carrageenan induced paw oedema model ,curcumin pyrazole analogue revealed the anti-inflammatory activity of 55.4% inhibition of paw edema  at 100mg/kg  and 67.69 % inhibition at 200mg/kg  and it was compared with the standard drug  dexamethasone .The  DPPH assay shown that there was a 91.8% inhibition of  DPPH radicals and it was compared with ascorbic acid and curcumin. The acute toxicity examination also evidenced that oral administration of 2000 mg/ Kg of curcumin pyrazole analogue doesn’t shown any mortality or behavioral changes and histological examination revealed normal architecture with no significant adverse effects on the vital organs. The results suggested that the curcumin pyrazole analogue was a very potent anti-oxidant and anti-inflammatory agent.

Published

2023-04-17

Issue

Section

Articles