Antioxidant, Hepatoprotective Activity And Anti- Inflammatory Of Agaricus Campestris (White Rot Fungi) Extract Against Diclofenac-Induced Hepatic Damage In Wistar Rats

Abstract

Agaricus campestris(AC) was used as traditionally medicine in treatment of diabetic liver disease, high cholesterol, and digestive issues in India, but its efficacy is uncertain. Folklore, on the other hand, has provided us with many effective plant-based therapies. The current research examined the role of CH3OH extract of AC (AEAC), in hepatoprotective and antioxidant activity. These fungi had the ability to inhibit the free radicals that are produced from the metabolism of toxic substance DFS. It also reduced the increase in serum aspartate amino transferase, alanine amino transferase, alkaline phosphatase, and total serum bilirubin caused by diclofenac sodium, while increasing lipid peroxidation and glutathione activities in the liver. To supplement the evidence from the biochemical analysis, a histopathological examination of the liver was previously performed. The anti- inflammatory (AI) activity of Hyaluronidase inhibition method was adopted. The results showed that AEACsignificantly (p<0.005) inhibit hyaluronidase. The presence of bioactive compounds like flavonoids, tocopherol and polyphenols revelation that AEAC has a significant protective effect against acute hepatotoxicity caused by diclofenac