Protective Effect of Liraglutide From Acute Renal Injury During Endotoxemia in Mice Mode

Abstract

. One of the most complex clinical challenges facing medical practice is sepsis-induced renal dysfunction, which results from polymicrobial sepsis and despite many therapeutic approaches have been used
in such clinical challenges, still there is more need for a new effective therapeutic approach. This study was
undertaken to investigate the protective effect of liraglutide from acute renal injury during endotoxemia in
mice and the mechanism behind this effect. 24 adult male Swiss-albino mice aged 8-12 weeks, with a weight
of 20-40 g, were randomized into 4 equal groups (n = 6), sham (laparotomy without CLP), sepsis (laparotomy
with CLP), vehicle (equivalent volume of NS), liraglutide (0.2 mg/kg/day s.c) group. Blood sample used to
assess serum levels of urea and creatinine. Kidney used to assess tissue TNF-α, IL-10, IL-6, TNFR1, TLR4
and TLR2 as well as the histological examination. Liraglutide caused significant improvement in renal function biomarker (serum level of urea, creatinine) and significant reduction in renal tissue level of TNF-α, IL6, TNFR1, TLR4 and TLR2 .In addition, liraglutide significantly increase the anti-inflammatory mediater
IL-10 in renal tissue. In histopathology examination, liraglutide significantly reduced the kidney injury and
minimize severity of tubular damage and inflammation in comparison with sepsis and vehicle groups that
showed severe renal injury, tubular damage and inflammation. Liraglutide can attenuate acute kidney injury during CLP-induced polymicrobial sepsis in mice.