Effect of Prunin on 3T3L1 Mouse Adipocytes Differentiation in Obesity

Abstract

Diabetes is a condition of high glucose content in blood which leads to several other complications like obesity, cardiac irregularities and artery problems. The objective of present study to evaluate
anti diabetic, anti-obesity and antioxidant properties of prunin through different in vitro assays. Prunin
was evaluated for various in vitro tests to access the therapeutic effect against adipogenesis in 3T3L1 mouse
adipocytes and different enzyme inhibition assays. The cytotoxicity of prunin was also determined by the
cell proliferation assay. Prunin showed dose dependent reduction in triglycerides content in an assay. The
treatment in differentiated adipocytes showed an increased (p =0.0169) lipolysis as measured by the release
of glycerol. Prunin did not exhibit any cytotoxic effect on adipocytes. The study concludes that prunin inhibits lipogenesis in adipocytes, antagonizes PPAR-γ and other lipogenic factors. It also accelerates triglyceride
mobilization from the fat cells or enhances lipolysis. The other results different in vitro enzyme inhibition was
also found significant effective and showed inhibition of enzymes involved in obesity and diabetes.