Formulation And Physico-Chemical Characterization Of Berberine And Rutin Loaded Chitosan Nanoparticles

Abstract

Every year, cancer threatens the lives of millions of people all over the world. We developed berberine and rutin-loaded nanoparticles using chitosan for treatment of cancer in the present research. Chitosan is safe, non-toxic and recyclable biopolymer that has found applications in biomedicine. Berberine and rutin seem to be natural polyphenolic compounds with a wide range of therapeutic properties. As a starting point, this work attempted to fill both of these natural chemicals into a homogeneous nanoparticulate system to improve biological availability and effectiveness. Dynamic light scattering (DLS), Scanning Electron Microscopy (SEM) were used to scan the particle size and shape of this unique formulation, which was prepared utilizing the ionic gelation process. Spectrometric studies of entrapment efficiencies and in vitro drug release studies were conducted. The therapeutic efficacy against cancer was evaluated on EAC cancer cell lines through MTT assay. Encapsulation efficacy of berberine and rutin loaded chitosan nanoparticles was observed about 40 80%. The reduction of EAC cell line was observed at 6.25 µg/ml and IC50 value is 19.33. According to the findings, incorporating berberine and rutin onto chitosan polymer nanoparticles is far more effective. Normal mammalian cells do not respond negatively to this formulation. Encapsulating effective herbal anti-cancer compounds, to put it another way, can be a good and promising technique.