Six Weeks Of HFD And A Single Low Dose Of STZ Administration Had A Massive Effect On The Biochemical And Pathological Profiles In Wistar Albino Rats

Abstract

Introduction: T2DM is a complicated disease, a variety of ailments that has been connected to dietary factors, genetic abnormalities, and lifestyle factors. To understand the aetiology of T2DM and to find new antidiabetic medicines, an accessible experimental animal model is required. Methods: 32 male Wistar rats are procured at the age of 9 weeks were utilized for the modeling of experimental T2DM After a 42 days of feeding a 60% HFD (60% of calories from lipids) and a 40 mg/kg-bw,a low dose of i.p. streptozotocin injection. In order to test the utilizing T2DM model, we measured the concentrations of insulin, triglycerides, total cholesterol, and glucose the fasting blood. One variable was used to contrast four groups using the Student's t-test. Multiple comparisons against the control group were made using a one-way ANOVA followed by a Dunnett post hoc test. Results: Hyperglycemia (p<0.001), hyperinsulinemia (p<0.001), dyslipidemia (p<0.001), and impaired glucose and insulin tolerance (p<0.001), were replicated in this study. This is a key metabolic abnormality of T2DM. As a result, we were able to diagnose and prognose , that may be used to evaluate antidiabetic medications. Conclusion: We were able to show that plasma glucose and basal total cholesterol impairment in streptozotocin-treated rats on a high-fat diet necessitate close monitoring of these biochemical indicators by comparing our results to those of other authors.