Design of Polylactic acid Nanoparticles by use of Central Composite Factorial Design Loaded with an Anti-inflammatory Drug

Abstract

In the current investigation, astragalin-loaded polymeric Nanoparticles were synthesized and optimised in an effort to increase drug delivery. A central composites factorial approach was utilised in order to investigate the ways in which various formulation elements influenced the zeta potential of polymeric nanoparticles, as well as their size and their capacity to effectively encapsulate other molecules. In both the equations based on polynomials and the response graphs, there was substantial evidence to establish the existence of a link among the dependent responses and one or more independent factors. The amount of polymer present, the length of time that the mixture is stirred, and the proportion of aqueous to organic components have each an effect on the size of the particles, zeta potential, then and encapsulation efficacy of polymeric nanoparticles. The optimal conditions were a 50 mg/ml polymer concentration, an hour of vigorous stirring, and a 5:1 aqueous to organic ratio. Zeta potential is -26 1.5 mV, encapsulation efficiency is 90 2.4%, and particle size is 122 2.2 nm. Stability tests on the optimised formulation are used to determine the physical and chemical stability of the polymeric nano formulation.