Invitro cytotoxic activity of Vitexin isolated from roots of V. negundo against MCF-7 cell lines

Abstract

Vitex negundo is commonly known as Chinese Chaste tree or five-leaved chaste tree. It is found mainly near water bodies, grasslands and forest areas. V.negundo found to possess analgesic, anti-oxidant, anti-inflammatory, anti-arthritic, anti-hyper pigmentation, immuno-stimulant, hepato-protective, anti-androgenic, pesticidal, anti-cancerous activity.  Studies evident that V.negundo contains ample bioactive leads that might be pharmacologically beneficial. The present study was aimed to find out the cytotoxic activity of Vitexin against MCF-7 Cell lines. The present study also focuses on screening, isolation and characterization of bioactive compound Vitexin. Vitexin is a pharmacologically beneficial flavonoid found to be in the root extract of V.negundo. The roots of V.negundo were subjected to qualitative and quantitative phytochemical screening. The root extract was further subjected to preparative Thin Layer Chromatography (pTLC), Liquid Chromatography Mass Spectrophotometry (LCMS), for the screening of flavonoids. The structure of Vitexin was confirmed by 13C NMR characterization analysis on comparison with the literature. The present work may develop simple and reliable method for the purification of bioactive flavonoids from the methanolic root extract of the medicinal plant V.negundo which may possesses abundant biomedical importance against cancer and inflammatory diseases. The cytotoxic activity of Vitexin was determined using MTT assay and the IC50 value was also calculated.