Biosynthesis of Silver Nanoparticles from Methanolic Extract of Cassia alata L and its Antibacterial activity

Abstract

As we know that Silver nanoparticles (SN) are very commonly found to be associated with a convalescent by inhibiting bacterial growth with fewer adverse effects like development of bacterial resistance and toxicity to the living cells. Methanolic extracts of Cassia alata L has been prepared in different concentrations purely as well as in combination with silver nanoparticles developed using Aspergillus flavus which were further tested against the isolates of both gram positive and gram negative bacteria using disc diffusion methods and minimal inhibitory concentrations. Various pathogenic bacteria like Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Shigella and Staphylococcus aureus were utilized to carry out this research work. Chemical profiling of the methanolic extract of Cassia alata L has been done using LC-MS. A symbiotic effect observed by both Cassia alata L methanolic extract as well as silver nanoparticles against all tested microbes. Pseudomonas aeruginosa has the lowest IFA values (0.10-0.13) while comparing with other strains and it doesn’t show any symbiotic activity. When used separately to both the silver nanoparticles and the Cassia alata L methanolic extract shows highest sensitivity with inhibition zones of 16 and 19 mm respectively. Strongest symbiotic effect has shown by silver nanoparticles and Cassia alata L methanolic extract against Staphylococcus aureus when 1 fold rise has occurred. In the present cutting edge medications, novel formulations can be prepared by using combination of silver nanoparticles with various medicinal plant extracts.