Chondroitin Sulphate Modified Silver Nanoparticle for The Delivery of Usnic Acid for Site Specific Targeting towards Breast Cancer Cells

Abstract

Introduction: The development of innovative nanomedicine platforms holds immense potential for enhancing cancer treatment efficacy while minimizing off-target effects.

Aims and Objectives: In the pursuit of enhanced breast cancer treatment strategies, this study presents a novel approach involving chondroitin sulfate modified silver nanoparticles (CS-AgNPs) for the site-specific delivery of Usnic Acid (UA). By combining the unique properties of silver nanoparticles and the specificity of chondroitin sulfate, a sophisticated delivery system is engineered.

Method: For this firstly, the 0.01 M silver nitrate (AgNO3) solution was prepared. After then the CS dispersion (5mg/mL) was prepared by dissolving 50 mg of CS in 10 mL of double distilled water. Subsequently The CS–AgNPs was synthesized by the reduction of Ag+ ions in aqueous medium using CS as both reducing and stabilizing agent. The CS was incubated with AgNO3 to allow Ag+ to bind to the carboxyl group of CS backbone, which is resulted in the solution immediately changing from colorless to yellowish brown, which indiacted the formation of CS-AgNPs. Furthermore, 10 mL of UA dispersion (3mg/mL) was added for loading the UA in CS-AgNPs and then centrifuged at 10000 rpm for 30 min to synthesis of CS-AgNPs-UA conjugate. Which was further measure the FTIR, NMR, particles size and zeta potential, drug release, drug loading, MTT assay, p53 and mTOR assay.

Result: The synthesized CS–AgNPs-UA nano-system possess a potent anticancer activity against breast cancer cell with all concentration i.e. 20ug, 40ug, 80ug and 160ug. It also suggested to be that the particles having nanometric size range (82nm) with zeta potential (-13.2mV), and round/globular in shape as per AFM analysis. The system was also hemocompatible, and release drug in sustained manner with 97.87% for prolonged time duration (96 hrs). 

Conclusion: The overall findings suggested that the system could be a potent candidate for breast cancer targeting.