Analysis Of The In-Vitro Antioxidant Activity Of The Active Fractions Derived From The Extracts Of Aegle Marmelos And Pedalium Murex Leaves

Abstract

The leaves of Aegle marmelos and the plant Pedalium murex have been historically employed as a therapeutic intervention for gastrointestinal infections, exhibiting properties such as antidiarrheal, anti-inflammatory, anticancer, antidiabetic, and antioxidant effects in humans. The extraction process involved the utilization of ethanol and subsequent fractionation through column chromatography utilizing various solvents, namely chloroform, ethyl acetate, methanol, and water. The invitro antioxidant activity, total flavonoid concentration, and total polyphenol content of nine fractions of Aegle marmelos and Pedalium murex leaf extract were evaluated using a spectrophotometric technique. The fractions recovered from the extraction process were determined based on the concentration of the solvent, resulting in a total of nine fractions. The fractions were assessed for their total antioxidant capacity, expressed in mg/ml of ascorbic acid. The range of values obtained for Aegle marmelos was 48-132 mg/ml, while for Pedalium murex it was 43.3-116.3 mg/ml. The total flavonoid content of the fractions was measured in terms of quercetin equivalent (QE), with values ranging between 47.5-83.5 mg/ml for Aegle marmelos and 112.6-146.3 mg/ml for Pedalium murex. Additionally, the total phenolic content of the fractions was determined in terms of Gallic acid equivalent (GAE), with values ranging between 108.2-181.5 for Aegle marmelos and 152.6-211.5 for Pedalium murex. Among the many fractions examined, it was revealed that the methanol water fraction exhibited superior antioxidant properties.