Tretinoin loaded miceller gel binding on α-β- γ retinoic acid receptor for the non-melanoma skin cancer management

Abstract

The purpose of the study was to examine drug-loaded polymeric micelles for topical administration systems and to improve Tretinoin penetration. Thin Film Hydration is used to create drug-loaded polymeric micelles. The drug content, particle size, in-vitro, ex-vivo, in-vivo skin penetration, in-vivo skin irritation, histopathology, and other criteria used to characterize polymeric micelles. Tri-block copolymer, specifically Pluronic-F 127, was employed in drug-loaded micelles. In order to create an optimised formulation, various formulations (F1–F12) had specific parameters changed. To create pH-sensitive micelles loaded miceller gel for topical distribution, formulation F7 was chosen because it had the smallest particle size of 83.12 nm, the lowest polydispersity value of 0.230, the highest drug content of 98.44±17%, and the highest cumulative drug permeation of 96.33±0.23% in 10 h. A comparative Ex-vivo skin permeation research between G1, F7, pure medication, and market preparation was carried out utilising pig skin employing five formulations (G1-G5) of miceller gel. Examined formulations' propensity to reach the desired concentration. When compared to market preparation, pure drug suspension, the optimised miceller gel demonstrated improved Ex-vivo skin penetration, displaying 67±0.37% permeability with zero-order kinetics (R2 =0.9971) over pig skin. Transmission Spherical polymeric micelles that were confined in the gel matrix were visible by electron microscopy. The range of trials demonstrated a gel formulation that was histologically safe, capable of retaining moisture, and had good handling properties. Finally, the sustain release topical formulation was effectively created for the treatment of non-melanoma skin cancer and was capable of delivering medication into the skin via topical delivery.