Protective Effect of Nepitrin in CCl4 Induced Hepatic Injury in Rats

Abstract

Y. The aim was to study the protective effects of nepitrin on liver function and lipid profile in rats.
The study was designed separately after inducing the hepatic damage by giving CCl4 (1.5 mL/kg of body
weight, i.p.) in separate group’s treatment in 14 days followed by 28 days separately. The rats were separated
into eight groups, each with six rats. The control group, I given water for 14 (Gr-Ia) and 28 (Gr-Ib) days,
respectively and group II received one-time dosage of CCl4 (1.5 mL/kg of body weight, i.p.) and divided
separately for 14 (Gr-IIa) and 28(Gr-II b) days study respectively. Nepitrin (150 mg/kg) was given orally to
groups III and IV for 14 and 28 days, respectively. Nepitrin (300 mg/kg) was given orally to groups V and
VI for 14 and 28 days, respectively. Blood was taken by heart puncture and examined for liver parameters
(ALP, ALT and AST) and lipid profile. In addition, the rat’s liver were collected and processed for histological studies. The results indicated a significant (p < 0.05) rise in alanine transaminase (ALT) and alkaline
phosphatase (ALP) levels in rats treated with Nepitrin (300mg/kg), but not in aspartate transaminase (AST),
total protein, or albumin. In the 14 and 28 day treated rats, total cholesterol, triglyceride, low density lipoprotein-cholesterol (LDL-C), and atherogenic index all significantly decreased and dose-dependently. In the
14 and 28-day rats treatment, high density lipoprotein–cholesterol (HDL-C) increased compared to control.
In addition, rat body weights were reduced over time. Results showed that nepitrin has hypolipidemic and
hepatoprotective potential at a dose of 300 mg/kg, i.p in rats.