Preparation and Characterization of Topical Nano emulsion of Rifampicin and Eucalyptus Essential Oil with Study of in Vitro Anti-Microbial Activity

Abstract

Objective(s):  Eucalyptus essential oil plays a significant role in the pharmaceutical, medicine industries having antimicrobial as well as antioxidant properties. The stability of these Nano emulsions was meticulously assessed over a span of 120 days. The instability of eucalyptus oil under typical conditions can lead to a loss of its bactericidal effectiveness. To safeguard this valuable natural product, the utilization of Nano-based emulsion technology emerges as an exceptional approach.

This study's goal was to create a topical nano emulsion that could treat microbial skin infections.

Method: A total of five formulations were formulated, each containing varying combinations of rifampicin and eucalyptus oil. These formulations were labeled as F1, F2, F3, F4, and F5. Employing Tween 80, and water through a sonication technique, Nano emulsions for all five formulations were created.

Various physicochemical attributes, including pH, viscosity, and antimicrobial efficacy, were investigated. The stability of these nanoemulsions was meticulously assessed over a span of 120 days.

Results: Nanoemulsions formulated using a combination of Tween 80, eucalyptus oil and rifampicin exhibited smaller particle sizes. The stability of the developed Nano emulsions persisted over a 120-day period, as evidenced by the absence of noteworthy alterations in pH, conductivity, and droplet size. Notably, nanoemulsions displayed significant in vitro antimicrobial efficacy.

Conclusion: The results indicated that rifampicin and eucalyptus essential oil-based nanoemulsions could be exploited as important medication carriers in the pharmaceutical industry.