Role of Yellow Wine in Homocysteine-induced Endothelial Progenitor Cell Function

Abstract

The key case change of coronary atherosclerosis (CHD) is atherosclerosis, and endothelial dysfunction plays a crucial role in the occurrence and development of atherosclerosis. The purpose of this study is to determine whether yellow wine and red wine can improve the function of endothelial progenitor cells (EPC) induced by homocysteine. Observe whether yellow wine and red wine can improve the characteristics of endothelial progenitor cells (EPCs) induced by homocysteine (Hcy). Extract rat bone marrow from bone marrow cell suspension using density gradient centrifugation to isolate monocytes and induce their differentiation into EPC. Divide the sample into 5 groups, including the control group. Western Blot detects the production of eNOS, p-eNOS, and NO. MTT and Transwell methods were used to detect endothelial progenitor cell apoptosis and in vitro angiogenesis. Compared with the control group, the proliferation, migration, and in vitro angiogenesis potential of EPCs significantly decreased after Hcy intervention (P<0.01). The production of eNOS, NO, and P-eNOS was significantly reduced (P<0.01). The intervention of yellow wine and red wine significantly improved the effect of Hcy on EPCs (P<0.01). Compared with the control group, the alcohol group had no significant change in the function of endothelial progenitor cells (P>0.05) and had no effect on endothelial cell apoptosis function (P>0.05). Hcy significantly reduces the proliferation, migration, and in vitro angiogenesis ability of endothelial progenitor cells, and reduces the production of eNOS, P-eNOS, and No. Both low doses of yellow wine and red wine can improve endothelial progenitor cell function.