Development And In Vitro Assessment Of Norfloxacin In Situ Ophthalmic Gel

Abstract

Topical anti-infectives are commonly employed for the treatment of bacterial conjunctivitis and corneal infections caused by susceptible bacterial strains like S. aureus, S. epidermidis, S. pneumoniae, and Enterobacter cloacae. These anti-infectives are traditionally administered into the eyes using formulations such as eye drops, eye ointments, and other conventional methods. However, they are often associated with issues such as rapid flushing, irritation, and inadequate retention within the eye. To address these shortcomings, a new formulation called in situ gel has emerged, offering a solution for the effective application of anti-infective agents via the ophthalmic route.

In this research, an in situ gel of Norfloxacin was developed, utilizing various grades of Hydroxypropyl Methyl Cellulose and Carbopol 934. Multiple in situ gel formulations were created and subjected to a thorough evaluation, encompassing assessments of visual appearance, clarity, pH, in vitro gelation, rheological properties, drug content analysis, and in vitro drug release. A total of eight formulations were developed, and upon optimization, Formulation F8 exhibited excellent gelling strength, clarity, acceptable pH, and improved in vitro drug release. This formulation is deemed promising as an in situ gel for Norfloxacin application.